Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time

S. Bagchi, B.G. Tellez, H.A. Rao, R. Lamendella, P.E. Saikaly
Applied Microbiology and Biotechnology, Volume 99, Issue 5, p. 2361-2370, (2015)

Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time

Keywords

Activated sludge, Bacterial diversity, Bacterial community dynamics, Rare taxa, 16S rRNA gene pyrosequencing

Abstract

​In this study, 16S rRNA gene pyrosequencing was applied in order to provide a better insight on the diversity and dynamics of total, dominant, and rare bacterial taxa in replicate lab-scale sequencing batch reactors (SBRs) operated at different solids retention time (SRT). Rank-abundance curves showed few dominant operational taxonomic units (OTUs) and a long tail of rare OTUs in all reactors. Results revealed that there was no detectable effect of SRT (2 vs. 10 days) on Shannon diversity index and OTU richness of both dominant and rare taxa. Nonmetric multidimensional scaling analysis showed that the total, dominant, and rare bacterial taxa were highly dynamic during the entire period of stable reactor performance. Also, the rare taxa were more dynamic than the dominant taxa despite expected low invasion rates because of the use of sterile synthetic media.

Code

DOI: 10.1007/s00253-014-6134-4

Sources

Website PDF

See all publications 2015