S. Bagchi, B.G. Tellez, H.A. Rao, R. Lamendella, P.E. Saikaly
Applied Microbiology and Biotechnology, Volume 99, Issue 5, p. 2361-2370, (2015)
Bacterial diversity, Bacterial community dynamics,
16S rRNA gene pyrosequencing
In this study, 16S rRNA gene pyrosequencing was applied in order to
provide a better insight on the diversity and dynamics of total,
dominant, and rare bacterial taxa in replicate lab-scale sequencing
batch reactors (SBRs) operated at different solids retention time (SRT).
Rank-abundance curves showed few dominant operational taxonomic units
(OTUs) and a long tail of rare OTUs in all reactors. Results revealed
that there was no detectable effect of SRT (2 vs. 10 days) on Shannon
diversity index and OTU richness of both dominant and rare taxa.
Nonmetric multidimensional scaling analysis showed that the total,
dominant, and rare bacterial taxa were highly dynamic during the entire
period of stable reactor performance. Also, the rare taxa were more
dynamic than the dominant taxa despite expected low invasion rates
because of the use of sterile synthetic media.